Mineral mining in the Fare East Federal District of Russia in the last century has resulted in large-scale disturbance of lands withdrawn from the forest resources and in huge accumulation of tailings. Extensive anthropogenic attack on the eco-sphere governs the stressful ecological situation in the study area in the vicinity of mines. Tailings storages containing large quantities of toxic waste are out of control. Located nearby residential areas, the tailings storages affect the health and environment. In connection with this, the research was aimed at development of reclamation approaches to the tailings storage at the closed mine of Khrustalny Mining and Processing Plant using biological systems (bio remediation) targeted at ecological safety of toxic waste and at improvement of the environmental conditions. To this effect, the objectives formulated include: 1. Analysis and generalization of the existing experience in recovery of land productivity at the tailings storages in Russia and abroad; 2. Estimation of anthropogenic impact on the eco-sphere; 3. Recommendations on reduction of aggravating toxic effect of waste on the environment. The revealed features of the anthropogenic pollution of the environment are the extremely high contents of toxic compounds of heavy metals (Zn, Pb, Cu, Ni, Cr, Co, Hg) and arsenic. The experimental research proves efficiency of using potential of biological systems in reclamation of surface of tailings ponds containing toxic processing waste. The novelty of the proposed methods is proved by patents of the Russian Federation.

Acknowledgements: The study was supported y the Russian Science Foundation, Project No. 1517-10016, and by the Russian Foundation for Basic Research, Project No. 18-35-00260.

For citation: Krupskaya L. T., Golubev D. A., Rastanina N. K., Filatova M. Yu. Reclamation of tailings storage surface at a closed mine in the Primorsky Krai by bio remediation. MIAB. Mining Inf. Anal. Bull. 2019;(9):138-148. [In Russ]. DOI: 10.25018/0236-1493-2019-09-0-138-148.


Tailings storage, reclamation, bio remediation, closed mine, anthropogenic pollution, heavy metals, mining waste, biological system potential.

Issue number: 9
Year: 2019
ISBN: 0236-1493
UDK: 622.235.3
DOI: 10.25018/0236-1493-2019-09-0-138-148
Authors: Krupskaya L.T., Golubev D. A., Rastanina N. K., Filatova M. Yu.

About authors: L.T. Krupskaya (1,2) — Dr. Sci. (Biol.), Professor, Honored Ecologist of Russia, Chief Researcher, e-mail:, D.A. Golubev (1,2) — Cand. Sci. (Eng.), Lecturer, Senior Researcher, N.K. Rastanina (1) — Cand. Sci. (Biol.), Assistant Professor, M.Yu. Filatova (1,2) — Graduate Student, Junior Researcher, 1) Pacific National University, 680035, Khabarovsk, Russia, 2) Far East Forestry Research Institute, 680020, Khabarovsk, Russia. Corresponding author: L.T. Krupskaya, e-mail:


1.        Vernadskiy V. I. Biosfera i noosfera [Biosphere and noosphere], Moscow, Nauka, 1989, 265 p.


2.        Kolesnikov B. P., Motorina L. V. Metody izucheniya biogeotsenozov v tekhnogennykh landshaftakh. Programma i metodika izucheniya tekhnogennykh biogeotsenozov [Methods for studying biogeocenoses in technogenic landscapes], Moscow, 1978, pp. 5—12. [In Russ].


3.        Gorovaya A. I. Methodological aspects of mutagenic background and genetic risk for humans and biota from the action of mutagenic environmental factors. Tsitologiya i genetika. 1996. Vol. 30(6), pp. 78—86. [In Russ].


4.        Zvyagintsev D. G. Metody pochvennoy mikrobiologii i biokhimii [Methods of soil microbiology and biochemistry], Moscow, Izd-vo MGU, 1991, 304 p.


5.        Beiyuan J., Awad Y. M., Beckers F., Tsang D. C., Ok Y. S., Rinklebe J. Mobility and phytoavailability of As and Pb in a contaminated soil using pine sawdust biochar under systematic change of redox conditions. Chemosphere, 2017, Vol. 178, pp. 110—118. DOI: 10.1016/j.chemosphere.2017.03.022.


6.        Chen M., Xu P., Zeng G., Yang C., Huang D., Zhang J. Bioremediation of soils contaminated with polycyclic aromatic hydrocarbons, petroleum, pesticides, chlorophenols and heavy metals by composting: Applications, microbes and future research needs. Biotechnology Advances, 2015, Vol. 33, pp. 745—755. DOI: 10.1016/j.biotechadv.2015.05.003.


7.        Yuan Y., Bolan N., Prévoteau A., Vithanage M., Biswas J. K., Ok Y. S., Wang H. Applications of biochar in redox-mediated reactions. Bioresource Technology, 2017, Vol. 246, pp. 271—281.


8.        Kushwaha A., Rani R., Kumar S., Gautam A. Heavy metal detoxification and tolerance mechanisms in plants: Implications for phytoremediation. Environmental Reviews, 2015, Vol. 24, pp. 39—51. DOI: 10.1139/er-2015-0010.


9.        Kang C.-H., Kwon Y.-J., So J.-S. Bioremediation of heavy metals by using bacterial mixtures. Ecological Engineering, 2016, Vol. 89, pp. 64—69.


10.     Silva P., Matos M. Assessment of the impact of aluminum on germination, early growth and free proline content in Lactuca sativa L. Ecotoxicology and Environmental Safety, 2016, Vol. 131, pp. 151—156. DOI: 10.1016/j.ecoenv.2016.05.014.


11.     Krupskaya L. T., Zvereva V. P., Golubev D. A., Bubnova M. B., Tagirova V. T. Problems of Reducing Environmental Damage Caused in the Past Century by Mining Facilities and Ways of Their Solution in the Far Eastern Federal District. Russian Journal of General Chemistry, 2017, Vol. 87 (13), pp. 3087—3094. DOI


12.     Krupskaya L. T., Zvereva V. P., Gula K. E., Gul’ L. P., Golubev D. A., Filatova M. Yu. Industrial wastewater treatment using higher aquatic vegetation in the former mining company of the Far Eastern Federal district. IOP Conf. Series: Materials Science and Engineering, 2017, pp. 1—6. DOI: 10.1088/1757-899X/244/1/012027.


13.     Krupskaya L. T., Cherentsova A. A., Mayorova L. P., Golubev D. A. Assessment of the environmental hazard class to waste accumulated over the past century by a former mining enterprise at the Far East Federal district. Russian Journal of General Chemistry, 2016, Vol. 86 (13), pp. 2983—2986. DOI


14.     Epov M. I., Yurkevich N. V., Bortnikova S. B., Karin Yu. G., Saeva O. P. Determination of the composition of mining waste by geochemical and geophysical methods (using the example of the tailing dump of the Salairsky mining and processing plant. Geologiya i geofizika. 2017. Vol. 58, no 12, pp. 1944—1954. [In Russ].


15.     Bortnikova S., Bessonova E., Gaskova O. Geochemistry of arsenic and metals in stored tailings of a co-ni arsenide-ore, Khovu-aksy area, Russia. Applied Geochemistry, 2012, Vol. 27, No 11, pp. 2238—2250. DOI: 10.1016/j.apgeochem.2012.02.033.


16.     Usikov V. I., Ozaryan Yu. A., Bubnova M. B., Lipina L. N. Environmental assessment of the Komsomolsk mining region (Russia) according to remote sensing data. Litasfera. 2016, no 1 (44), pp. 114—121. [In Russ].


17. Bubnova M. B., Ozaryan Yu. A. Environmental monitoring of natural-mining systems based


on remote sensing data. Ekologicheskie sistemy i pribory. 2015, no 11, pp. 15—22. [In Russ].


18.     Bubnova M. B. The method of integral assessment of the level of environmental pollution in the operation of deposits of non-ferrous and rare metals. Izvestiya vysshikh uchebnykh zavedeniy. Gornyy zhurnal. 2006, no 4, pp. 86—91. [In Russ].


19.     Zvereva V. P., Frolov K. R., Pyatakov A. D. Simulation of sulfide oxidation processes at the tailings deposit of the Vysokogorskoe deposit at a temperature of from –25 to 0 °C (Kavalerovsky district of Primorsky Krai). Ekologicheskaya khimiya. 2018. Vol. 27, no 3, pp. 135—140. [In Russ].


20.     Krupskaya L. T., Kirienko O. A., Mayorova O. A., Golubev D. A., Onishchenko M. S. Patent RU 569582, 27.11.2015.


21.     Krupskaya L. T., Mayorova L. P., Orlov A. M., Zvereva V. P., Izotov D. V., Morin V. A., Leonenko A. V., Golubev D. A. Patent RU 2486733, 10.07.2013.


22.     Krupskaya L. T., Rastanina N. K., Golubev D. A., Filatova M. Yu. Patent RU 2672453, 14.11.2018.


23.     Krupskaya L. T., Leonenko N. A., Golubev D. A., Leonenko A. V. Patent RU 2625469, 14.07.2017.

Subscribe for our dispatch